 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 5]](nw5071fig5mag.jpg)
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Figure 5
(a) Stereoview of detailed interactions between the ZRANB3 APIM (yellow) and PCNA (cyan). The IDCL of PCNA is shown in blue as in Fig. 2 ![[link]](../../../../../../logos/arrows/f_arr.gif) (a) and the left panel of Fig. 2(c). Electrostatic interactions are shown as grey dots. Residues of the ZRANB3 APIM and PCNA are labelled in yellow and black, respectively. (b) Schematic drawing of van der Waals interactions of the ZRANB3 APIM with PCNA. The ZRANB3 APIM and the ICDL of PCNA are shown as cartoon representations. The colours correspond to those in Fig. 2(a). Cyan bleeding indicates van der Waals interactions with the surrounding residues of PCNA indicated in boxes. (c) Interaction analysis between the ZRANB3 APIM and PCNA. The double dagger is defined in the legend of Fig. 3. Lane 1 contains PCNA only. Lane 2 contains GST and PCNA, showing no interaction between GST and PCNA. Lane 3 contains wild-type GST-ZRANB3(861–1079) as a positive control (CTL). Lanes 3–17 contain GST-ZRANB3(861–1079) with single or double mutations. FL/AA indicates the F1075A/L1076A double mutant of GST-ZRANB3(861–1079). The relative band intensities of PCNA are shown as in as Fig. 3 (n = 3; Dunnett's test; *, p < 0.05; ***, p < 0.001). |
![[Figure 5]](nw5071fig5.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
