 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](ek5007fig2mag.jpg)
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Figure 2
SDS–PAGE and isoelectric focusing (IEF) and in-gel activity staining of PNGM-1 using nitrocefin. (a) SDS–PAGE analysis of protein samples during purification. From left to right, molecular-weight markers (lane 1; labelled in kDa), whole-cell extract (lane 2), soluble protein (lane 3) and purified PNGM-1 (lane 4). (b) IEF and overlay assay of PNGM-1. Lane 1, pI marker (pH range 3–10; labelled); lane 2, silver staining after IEF of PNGM-1; lane 3, overlay activity assay with 100 mM nitrocefin solution after IEF of PNGM-1. |
![[Figure 2]](ek5007fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
