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Figure 3
Crystal structure of Asn149pNO2F sfGFP (PDB entry 6dq1). The overall structure is shown in cartoon representation in (a) with the Asn149pNO2F residue and chromophore shown in stick representation. The initial 2Fo − Fc map at 1.0σ and Fo − Fc map at +3.0σ and −3.0σ around the N149A site are shown in (b) in blue, green and red mesh, respectively. (c) The final 2Fo − Fc map at 1.0σ is shown in blue mesh around the pNO2F modeled at the Asn149 site. Figures were generated in PyMOL (Schrödinger). |