 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
journal menu
![[Figure 2]](rl5166fig2mag.jpg)
|
|
Figure 2
Redox manipulation of perdeuterated human MnSOD crystals via vapor diffusion within capillaries. Cracks were present prior to manipulation. (a) Images of crystals within capillaries along with deuterated reservoir supplemented with either potassium permanganate for oxidation or dithionite for reduction. (b) Time course of crystal reduction using reservoir solutions supplemented with 1% hydrogen peroxide. The purple/pink background is from the microscope polarizer with its setting maintained between images, while the lighting was consistent between the images in this panel. (c) Complete reduction by hydrogen peroxide is observed by the absence of pink color that is achieved 72 h after supplementation with hydrogen peroxide. The image was taken with alternative lighting compared with (b) and without the polarizer to better observe the colorlessness. Diffraction quality was maintained during this time course (data not shown). (d) The same crystal was oxidized by supplementation with potassium permanganate and reduced with sodium dithionite. The purple background is from the microscope polarizer with its setting maintained between images. The lighting was kept consistent between the images. |
![[Figure 2]](rl5166fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
Open 
access
access
