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Figure 5
Effects of crystal size and dithionite-treatment method on MnSOD crystal integrity. (a) Crystals that were soaked in a deuterated substitute reservoir solution supplemented with 0.2 M dithionite for 3 d. The small specks appeared one week after removal of the soaking solution. These could be salt or protein aggregates. A full neutron data set was collected from the crystal shown in the left image owing to its size and lower mosaicity (judged by X-ray diffraction) compared with the other samples. (b) Crystals treated with dithionite by the touch-soak method using the same solution as in (a). The larger crystals in (a) re-oxidized from the reduced state when using the touch-soak method, which can be attributed to their ∼25% larger volumes compared with those in (b). (c) Images of crystals treated with 0.3 M dithionite by vapor diffusion. Arrows indicate aggregates on or within the crystals. The left image depicts a minor detriment from aggregates to the protein crystal quality, whereas the right image shows aggregate growth within the sample that significantly affects the quality of the protein crystal. |
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