Figure 5
Crystallization islands for six test proteins after ISO. Each crystallization condition in Fig. 2 that yielded crystals is plotted as a single dot according to the three mechanistically distinct precipitants within that condition: salt, high-molecular-weight PEG and organic solvent. The pH of each condition is indicated by the color of the dot according to the legend (bottom). Productive crystallization conditions cluster in different `crystallization islands' that range from single discrete points (e.g. bovine catalase at high salt, pH 6.5 in the upper left panel) to multi-condition clusters (e.g. mCherry with a broad range of PEG–organic conditions in the lower right panel). |