Figure 2
Particle angular distribution and map resolution. (a) The angular distribution of refined particle orientations indicates a predominance of views (red) perpendicular to the filament axis. (b) Fourier shell correlation plots indicated a resolution of 3.1 Å using a gold-standard FSC cutoff of 0.143 as indicated by the dashed line. The local resolution of the NP helical filament (c) and an isolated NP protomer (d) was estimated in RELION (Kucukelbir et al., 2014) and indicates a high-resolution folded core of NP with lower-resolution peripheral regions. |