 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](tb5139fig1mag.jpg)
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Figure 1
Protein preparation. (a) Fluorescence-detection size-exclusion chromatography (FSEC)-based analysis of His6 (black) and His8 (red) constructs. The histidine tag-specific fluorescent probe P3NTA was used for detection (excitation, 482 nm; emission, 520 nm). The peak corresponding to tetrameric PepTSo2 is marked with an asterisk. (b) SEC chromatogram of purified PepTSo2. The fluorescent signals, which were mainly derived from the tryptophan residues, were monitored by the fluorescence detector, with excitation at 280 nm and emission at 350 nm. The peak corresponding to tetrameric PepTSo2 is marked with an asterisk. (c) SDS–PAGE analysis with Coomassie Brilliant Blue staining. Left lane, molecular-weight markers (labeled in kDa); right lane, the merged peak fraction from the SEC purification. |
![[Figure 1]](tb5139fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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