Figure 1
Galactobiose binding site in chain A of AaGal. (a) Overview of the enzyme with galactobiose. The galactobiose and glycosylation are shown in stick representation (with yellow and green C atoms, respectively). Ca2+ ions are depicted as blue spheres and the polypeptide chain is shown as a grey ribbon. The N-terminus and C-terminus are labelled and are coloured cyan and red, respectively. (b) The difference map before modelling of galactobiose (Fobs − Fcalc map contoured at the 3.0σ level). (c) Electron density for galactobiose (weighted 2Fobs − Fcalc map contoured at the 1.0σ level). |