issue contents
June 2019 issue
![Highlighted illustration](/f/issues/2019/06/00/graphics/coverill.gif)
Cover illustration: Structure of the Prx6-subfamily 1-Cys peroxiredoxin from Sulfolobus islandicus [Stroobants et al. (2019), Acta Cryst. F75, 428-434].
research communications
The structure of Aspergillus aculeatus β-1,4-galactanase, a glycoside hydrolase belonging to family 53, has been determined in complex with galactobiose at subsites −1 and −2.
PDB reference: β-1,4-galactanase in complex with galactobiose, 6q3r
Crystal structures of the extracellular domain of rabbit neprilysin and of neprilysin complexed with the inhibitors phosphoramidon or thiorphan are reported.
The apo structure of the SPRY domain of human SPRY domain-containing SOCS box protein 2 (SPSB2) determined at 1.9 Å resolution shows that its inducible nitric oxide synthase (iNOS)-binding site is highly preformed and that the C-terminal His6 tag binds to a shallow pocket adjacent to the iNOS-binding site, which may help in structure-based and fragment-based SPSB2 inhibitor design.
PDB reference: human SPSB2 in the apo state, 6jkj
High-resolution structures of apocruzain and cruzain bound to S-methyl thiomethanesulfonate provide insights for the structure-based design of new cruzain inhibitors.
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The crystal structure of the 1-Cys peroxiredoxin from S. islandicus is presented; it assembles into a ring-shaped decamer composed of five homodimers. It is concluded that this Prx6-like protein undergoes decamerization independently of arm-domain cysteines.
PDB reference: 1-Cys peroxiredoxin, 6q5v
The growth of a large crystal of a potassium ion channel, and neutron and X-ray data collection from the crystal are described.
The dimerization domain of splicing factor proline/glutamine-rich (SFPQ), an essential RNA-binding protein, has been crystallized in the C-centered orthorhombic space group C2221 with one monomer in the asymmetric unit, and its structure has been determined and refined to 1.9 Å resolution. The crystal structure was analyzed and compared with the solution scattering data.
PDB reference: dimerization domain of human SFPQ in space group C2221, 6ncq
The crystal structure of the flavin-dependent thymidylate synthase Thy1 from Thermus thermophilus HB8 (TtThy1, TTHA1096) was determined in complex with FAD and phosphate at 2.5 Å resolution. TtThy1 is a tetrameric molecule, to which four FAD molecules are bound. Two phosphate ions were bound to each dUMP-binding site. The characteristic feature of TtThy1 is the existence of an extra C-terminal domain (CTD) consisting of three α-helices and a β-strand.
PDB reference: thymidylate synthase, 6j61
Interferon-inducible protein 204 (p204) binds to microbial DNA to elicit inflammatory responses and induce interferon production. The DNA-binding affinity of the p204 HIN1 domain has been characterized and its crystal structure has been determined. Surface-charge distribution together with sequence alignment suggests that the p204 HIN domain uses its L12 and L45 loops for DNA binding.
PDB reference: HIN1 domain of interferon-inducible protein 204, 6oe9
Human liver pyruvate kinase converts phosphoenolpyruvate to pyruvate in the final step of glycolysis and is allosterically activated by fructose-1,6-bisphosphate. The allosteric site is located between the phosphate-binding loop and the allosteric loop. The crystal structures of four site-directed mutants revealed a conformational toggle between the open and closed positions of the allosteric loop.