 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 4]](pg5083fig4mag.jpg)
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Figure 4
S. pombe U7BR impedes Ubc7 transthiolation. (a) Percentage of free Ubc7 remaining in a transthiolation assay with and without excess U7BR. Error bars represent one standard deviation. (b) E2–E2BR co-crystal structures from S. pombe (PDB entry 6op8), H. sapiens (PDB entry 3h8k) and S. cerevisiae (PDB entry 4jqu; Metzger et al., 2013  ) were modeled into the crystal structure of S. pombe E1–Ubc4–Ub–ATP (PDB entry 4ii2; Olsen & Lima, 2013) by superposing the E2 structures. The black square indicates the area displayed in (c). (c) The N-terminal portions of S. pombe U7BR and H. sapiens G2BR, but not S. cerevisiae U7BR, clash with the UFD domain of E1. IAD, inactive adenylation domain; AAD, active adenylation domain; Ub, ubiquitin; UFD, ubiquitin-fold domain; FCCH, first catalytic cysteine half-domain; SCCH, second catalytic cysteine half-domain. |
![[Figure 4]](pg5083fig4.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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