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Figure 3
Structure and assembly of EcDSCD. (a) Superposition (stereo figure with depth cue) of the 24-mer biological assemblies of EcDSCD in the F432 (sky blue; PDB entry 1scz) and I4 (red; this work) crystal forms with respect to monomer A. For clarity, only the top 12-mer is shown as ribbons. The physiological significance of the observed structural changes is currently unknown, and these structural differences may arise from a different molecular packing under our crystallization conditions. (b) Stereoview of the superimposition of monomer A in the F432 (blue) and I4 (red) crystal forms. Significant structural changes are present in some loops; however, the overall structures are very similar (r.m.s.d. of 0.3 Å). The structural differences seen in the 24-mer superpositions arise from the differences in these loop-mediated intermolecular interactions, as well as the intermolecular rotational and translational changes. (c) Stereoview of the superposition of the trimers in P3121 (PDB entry 1c4t, yellow–orange), F432 (PDB entry 1scz, sky blue) and I4 (PDB entry 6pbr, red) crystal forms. Some conformational changes exist in helices and loop areas.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 75| Part 9| September 2019| Pages 616-624
https://doi.org/10.1107/S2053230X19011488
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