|
Figure 2
(a) SDS–PAGE analysis of EsaAex_1 after limited proteolysis. Lane M contains molecular-mass markers (labeled in kDa). (b) Sequence of EsaAex_1: the peptide coverage detected by fingerprint mass spectrometry (red) indicates the protein boundaries of the excised band at 43 kDa. (c) Schematic of the full-length EsaA protein showing the EsxAex_1 and EsaAex_2 boundaries and the MS fingerprint (red). (d) SDS–PAGE of EsaAex_2 after size-exclusion chromatography. Molecular-mass markers are labeled in kDa. |
Open
access
