Enhanced X-ray diffraction of in vivo-grown μNS crystals by viscous jets at XFELs

Nagaratnam, N.; Tang, Y.; Botha, S.; Saul, J.; Li, C.; Hu, H.; Zaare, S.; Hunter, M.; Lowry, D.; Weierstall, U.; Zatsepin, N.; Spence, J.C.H.; Qiu, J.; LaBaer, J.; Fromme, P.; Martin-Garcia, J.M.

doi:10.1107/S2053230X20006172

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
Detection and characterization of EGFP-μNS_(448–605) crystallization in Sf9 cells. (a) EGFP-μNS_(448–605) crystals either inside Sf9 cells or released to the medium two days post-infection observed by DIC (left) and GFP fluorescence (right) microscopy with the same field of view. (b) UV fluorescence image of EGFP-μNS_(448–605) crystals in Sf9 cells (left) and SONICC image of high-density Sf9 cells harboring EGFP-μNS_(448–605) crystals (right) three days post-infection. (c) TEM images of an Sf9 insect cell with EGFP-μNS_(448–605) crystals grown inside (left), EGFP-μNS_(448–605) crystals with a hexagonal cross section (middle) and the lattice structure of the crystal (right). Crystals denoted by asterisks in the left panel are shown in the middle panel at a higher magnification. Particles surrounding the crystals (hypothesized to be ribosomes) are indicated by black arrowheads in the middle panel. The arrow in the right panel points to the crystalline lattice.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 76| Part 6| June 2020| Pages 278-289

https://doi.org/10.1107/S2053230X20006172

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