Enhanced X-ray diffraction of in vivo-grown μNS crystals by viscous jets at XFELs

Nagaratnam, N.; Tang, Y.; Botha, S.; Saul, J.; Li, C.; Hu, H.; Zaare, S.; Hunter, M.; Lowry, D.; Weierstall, U.; Zatsepin, N.; Spence, J.C.H.; Qiu, J.; LaBaer, J.; Fromme, P.; Martin-Garcia, J.M.

doi:10.1107/S2053230X20006172

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 2
EGFP-μNS_(448–605) crystals resuspended in PBS buffer. (a) EGFP-μNS crystals extracted from Sf9 cells by gentle sonication and resuspended in PBS buffer three days post-infection observed by DIC (left) and GFP fluorescence (right) microscopy with the same field of view. (b) SONICC images of high-density EGFP-μNS_(448–605) crystal pellets extracted from Sf9 insect cells two days (left) and three days (right) post-infection.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 76| Part 6| June 2020| Pages 278-289

https://doi.org/10.1107/S2053230X20006172

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