Enhanced X-ray diffraction of in vivo-grown μNS crystals by viscous jets at XFELs

Nagaratnam, N.; Tang, Y.; Botha, S.; Saul, J.; Li, C.; Hu, H.; Zaare, S.; Hunter, M.; Lowry, D.; Weierstall, U.; Zatsepin, N.; Spence, J.C.H.; Qiu, J.; LaBaer, J.; Fromme, P.; Martin-Garcia, J.M.

doi:10.1107/S2053230X20006172

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 5
Electron-density maps (2mF_o − DF_c) contoured at 1σ. (a) Ribbon representations of the two EGFP molecules in the asymmetric unit are shown without (left) and with (right) maps in the same orientation. (b) A 90° rotation of the EGFP molecules shown in (a). (c) Helical fragments running through the center of one of the EGFP molecules that coordinate the fluorescent chromophore are shown and highlighted by the black arrow. For clarity, the EGFP molecules from PDB entry 3lvc have been overlaid and are represented as a light gray cartoon in all panels shown.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 76| Part 6| June 2020| Pages 278-289

https://doi.org/10.1107/S2053230X20006172

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