 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](ir5012fig1mag.jpg)
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Figure 1
Gel-filtration profiles of EcGAPDH1. (a) Top: gel-filtration profile of EcGAPDH on a HiPrep 26/60 Sephacryl S-100 HR column. EcGAPDH eluted at 108 ml. Bottom: 12% SDS–PAGE of wild-type protein eluted from gel filtration. (b) Gel-filtration standards (Bio-Rad, California, USA) on a HiPrep 26/60 Sephacryl S-100 HR column. Bovine thyroglobulin, with a molecular weight (MW) of 670 kDa, eluted at 92.3 ml. Bovine γ-globulin (bovine), with a MW of 158 kDa, eluted at 104.6 ml. Chicken ovalbumin, with a MW of 44 kDa, eluted at 132 ml. Horse myoglobin, with a MW of 17 kDa, eluted at 171.3 ml. Vitamin B12, with a MW of 1.35 kDa, eluted at 286 ml. |
![[Figure 1]](ir5012fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
