 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](no5175fig1mag.jpg)
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Figure 1
Purification and characterization of TeCyt c6. (a) The purity of the protein was verified by SDS–PAGE. (b) Absorbance properties were characterized by UV–Vis absorbance spectroscopy; the cytochrome was oxidized by the addition of 1 mM potassium ferricyanide. (c) Additionally, MALDI-TOF MS indicates a dimeric state of the protein (19.6 kDa) as well as a trimeric state (29.4 kDa) (see also Supplementary Fig. S3). (d) In solution, homogenous oligomerization is indicated by bnPAGE in the presence and absence of 10 mM DTT at a protein concentration of 0.5 mg ml−1. Considering a nonglobular oligomerization state of the protein, the molecular-weight standard provides a rough mass estimation. Trimerization is further supported by IR-DLS (Supplementary Fig. S4). The molecular masses of the standard proteins are indicated in kDa. |
![[Figure 1]](no5175fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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