Figure 2
(a) Elution profile of RoMCP after passage through a Superdex 200 Increase 10/300 gel-filtration column. The retention volume of 13.7 ml corresponds to an estimated molecular weight of 60 kDa. The broken lines highlight the upper and lower exclusion limits of the column. The black bar indicates the fractions that were pooled and loaded onto an SDS–PAGE gel. (b) SDS–PAGE of purified RoMCP. Asterisks indicate the bands that were assigned to RoMCP and have apparent molecular weights of 20–25 and 80–85 kDa. (c) Monoclinic crytals of RoMCP. |