Figure 1
Purification and self-rotation function analysis of Rho. (a) Size-exclusion chromatograph of NaAtm1 purification using a HiLoad Superdex 200 16/60 column. The column void volume is colored gray, while the elution positions of various molecular-weight standards (in kDa) are marked on the chromatograph. (b) SDS–PAGE of the peak fractions of SEC purification from (a). (c) The χ = 180° and 60° sections of the self-rotation function calculated using MOLREP in CCP4 (Winn et al., 2011) with an integration radius of 51 Å using diffraction data between 3.3 and 40 Å resolution. |