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Figure 2
Time series of Gab2-pT391 replacing cJun-pS227 in the amphipathic groove of 14-3-3σ over (a) 1 h, (b) 24 h, (c) 48 h and (d) 96 h. Growth of electron density for the Gab2-pT391 peptide is observed over time. The peptide is represented by yellow sticks. 14-3-3 is represented by a white, solid surface. The Fo − Fc electron-density map (represented by a green mesh) around the peptide is contoured at 2.4σ. Refined occupancies for the modelled amino acids of the Gab2-pT391 peptides are plotted in Supplementary Fig. S1. |
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