 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 3]](ow5025fig3mag.jpg)
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Figure 3
(a) SDS–PAGE gels showing overexpression of native and SeMet SmhA. Lane 1, pre-induction insoluble fraction; lane 2, pre-induction soluble fraction; lane 3, post-induction insoluble fraction; lane 4, post-induction soluble fraction. (b) SDS–PAGE gels showing nickel HiTrap column purification of native and SeMet SmhA. Lane 1, cell-free extract; lanes 2–5/6, elution fractions from the nickel HiTrap column. Fraction 6 and fractions 4 and 5 (native and SeMet, respectively) were >90% pure and were used for crystallization. |
![[Figure 3]](ow5025fig3.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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