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Figure 2
FLL27D is a stable monomer capable of binding only one FLT3 molecule. (a) SEC-MALLS characterization of FLWT, FLL27D and receptor complexes thereof. Elution profile monitored by the forward and right-angle laser detector (left axis) plotted against the SEC retention volume and overlaid with the measured molecular weight (right axis). FLWT (green) is able to recruit two FLT3 molecules (yellow) into complex formation (blue), whereas FLL27D (gray) binds FLT3 in an equimolar fashion (red). (b) Summary of the predicted molecular weights, based on the amino-acid sequence, and the MALLS-measured molecular weights. Further glycoprotein conjugate analysis of the latter allowed part of the mass to be attributed to the glycan content.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 77| Part 4| April 2021| Pages 121-127
https://doi.org/10.1107/S2053230X21003289
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