 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](nj5303fig1mag.jpg)
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Figure 1
Structure and purification of full-length GABARAPL2. (a) The new crystal form of GABRAPL2 (PDB entry 7lk3) containing two molecules in the asymmetric unit. Chain A (CHA) is depicted in deep purple and chain B (CHB) in lilac. Overlay of (b) CHA from PDB entry 7lk3 with the equivalent chain of PDB entry 4co7 (orange) and (c) overlay of CHB from PDB entry 7lk3 (lilac) with CHB from PDB entry 4co7 (light orange). Regions of divergence in the protein backbone involving cleavage-artefact serine residues at the N-termini are shown (black arrows). (d) Size-exclusion chromatogram (SEC) of monomeric GABARAPL2 (GBRPL2) and SDS–PAGE gel of fractions stained with Coomassie Brilliant Blue. (e) A representative surface plasmon resonance sensorgram (top panel) and dose–response curve (bottom panel) for two independent experiments showing the binding of purified full-length GABARAPL2 by an NDP52 I133W LIR peptide (Super LIR) with Kd values of 4.85 and 5.07 µM. |
![[Figure 1]](nj5303fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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