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Figure 2
Differences in unit-cell dimensions between PDB entry 4co7 and the new crystal form of GABARAPL2 manifest in the P21 lattices due to a unique crystal contact. (a) An overlay of symmetry mates between PDB entry 4co7 (orange) and the new structure (purple) after performing a y + 1/2, −z symmetry operation to align the structures with the same origin reveals a slight offset in the positioning of CHB in the published structure. (b) Overlay of the crystal lattices of the new crystal form (purple) and PDB entry 4co7 (orange) in space group P21 exhibiting how the CHB offset manifests in the crystal form. Unit-cell dimensions are listed below. (c) A crystal contact between Arg28 and Glu12, showing the salt bridge that is bidentate in the untwinned PDB entry 7lk3 and monodentate in the published, twinned PDB entry 4co7 structure (see inlays for comparison). The interface occurs between two chains of the same type in PDB entry 7lk3 (lilac) and between chains A (orange) and B (light orange) in PDB entry 4co7.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
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