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Figure 5
Electron density in the solvent channels of cleaved and uncleaved ds-TrpR. An assembly of the cleaved Val58Ile ds-TrpR crystal structure is shown in (a) that was made in Coot (Emsley et al., 2010BB13) by expanding the single TrpR molecule in the asymmetric unit (red ribbons) with symmetry mates (gray ribbons) to cover the crystal solvent channel. Structural assemblies as in (a) are shown for cleaved and uncleaved Val58Ile ds-TrpR in the upper panels in (b) and (c), respectively. Electron density is presented as a 2FoFc map (blue) with 0.45 I/σ and map blurring with B2 = 133 Å2. The lower panels in (b) and (c) present magnified views of the channel area of the assemblies shown in the top panels. Relative to (a), the assemblies in (b) and (c) are shown rotated by 30° about the crystal 61 screw axis to more clearly show features of interest.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
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