Figure 2
Difference Fourier anomalous map and experimental electron-density map for the IRF4 DBD. (a) Difference Fourier anomalous map contoured at 5σ generated by Crank2 from the partially built model (no nucleic acids built). The grey electron density corresponds to P atoms from the DNA molecule, and the blue electron density, in the major DNA groove, to the S atoms from the IRF4 DBD. The weaker electron density at the very top of the picture corresponds to the S atom from the third IRF4 DBD molecule. (b) Experimental electron-density map generated by Crank2. The final model of the IRF4 DBD is fitted in the map to assess the map quality. This figure was prepared with PyMOL (version 2.0; Schrödinger). |