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Figure 1
Structure of CgGDE and its oligosaccharide-binding sites. (a) Structure of CgGDE (PDB entry 5d0f). Subdomains A, B and C in the GT domain, the GC domain and domains M1 and M2 in CgGDE are colored as indicated in the bar diagram. The previously identified oligosaccharide-binding sites are referred to as sites 1–5 and are indicated. (b, c) Oligosaccharide binding to the GT (b) and GC (c) active sites in CgGDE. The asterisk in (b) indicate the possible connection between the glycogen main chain and branch. The asterisk in (c) indicates the possible location of the branch. Catalytic residues and additional important residues at the binding sites are highlighted. (d, e) As in (b) and (c) with CgGDE presented in surface representation. Structural figures were prepared with PyMOL (https://www.pymol.org). |