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Figure 1
A simple and gentle method for H/D exchange for cryogenic neutron crystallography. (a) Start with a crystal immersed in a hydrogenated cryoprotectant. A deuterated counterpart is then added to the solution with gentle mixing, which results in the percentage of deuterated content increasing while the percentage of hydrogenated content decreases. Initially, modest volumes of deuterated cryoprotectant should be added to minimize damage to the crystals. (b) In preparation for further dilution of hydrogenated content, the volume of the solution is decreased. (c) Iterations of processes (a) and (b) lead to the near-complete depletion of hydrogenated solution and immersion of the crystal in nearly 100% deuterated cryoprotectant.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 78| Part 1| January 2022| Pages 8-16
https://doi.org/10.1107/S2053230X21012413
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