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Figure 3
Schematic of the flash-freezng process for large crystals and its key points. (a) Material setup prior to flash-freezing. Care should be taken in choosing an appropriate cryoloop diameter and in ensuring that both the crystal and a large portion of the cryoloop stem are well coated in cryoprotectant. In preparation for freezing, the crystal-containing cryoloop is mounted on the goniometer while an object (card) blocks the cryostream. (b) After mounting, quick removal of the card leads to a vitreous flash-freeze with the absence of crystalline ice.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 78| Part 1| January 2022| Pages 8-16
https://doi.org/10.1107/S2053230X21012413
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