Structural characterization of the urease accessory protein UreF from Klebsiella pneumoniae

Liu, S.; Wu, W.; Zhao, Q.; Liang, H.; Che, S.; Zhang, H.; Liu, R.; Zhang, Q.; Bartlam, M.

doi:10.1107/S2053230X22000474

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
(a) Circular-dichroism spectrum of KpUreF in the absence (black squares) and the presence (light blue circles) of Ni²⁺ ions. (b) Analytical ultracentrifugation profile of KpUreF showing a single peak at ∼45 kDa corresponding to the molecular mass of a dimer. A representative SDS–PAGE gel (stained with Coomassie Blue) is shown in the inset.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 78| Part 2| February 2022| Pages 75-80

https://doi.org/10.1107/S2053230X22000474

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