 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 3]](tb5174fig3mag.jpg)
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Figure 3
(a) Comparison of KpUreF (green/pale green) and HpUreF (cyan/pale cyan) dimer structures. (b) The KpUreF dimer structure, with one monomer shown in surface representation and coloured according to sequence conservation (as calculated by ConSurf: magenta, high sequence conservation; white, average sequence conservation; cyan, low sequence conservation). The other monomer is shown in cartoon representation and coloured green. The view on the left is in the same orientation as in (a). The view on the right is after rotation by 90° about the y axis. (c) The HpUreG and HpUreH subunits of the HpUreF–UreG–UreH ternary-complex structure (PDB entry 4hi0) modelled onto the KpUreF dimer following superposition of KpUreF and HpUreF. The KpUreF dimer is coloured according to (b). The structures of HpUreH (named UreD in K. pneumoniae) and HpUreG are shown in grey and labelled. The view on the left is in the same orientation as in (a). The view on the right is after rotation by 90° about the x axis. (d) A representative SDS–PAGE gel (stained with Coomassie Blue) of the purified KpUreF–UreD complex (lanes 1 and 3, KpUreF–UreD; lane 2, marker). |
![[Figure 3]](tb5174fig3.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
