Figure 1
DNA-binding modes of (a) the CENP-SX tetramer and (b) the FANCM–CENP-SX pentamer to dsDNA of various lengths. (a) EMSA of CENP-SX (1.25 µM) with 19, 25, 31, 37, 43, 49, 55, 61, 67, 73, 79, 85, 91 and 97 bp dsDNA (1.25 µM). (b) EMSA of FANCM–CENP-SX (1.25 µM) with the same set of dsDNAs (1.25 µM) as used in (a). Protein–DNA complex bands are numbered accordingly. |