Figure 1
Features, purification and crystallization of hROR1-KRD. (a) Domain organization of human ROR1. (b) SEC chromatogram of hROR1-KRD. (c) SDS–PAGE analysis of hROR1-KRD purification, showing the sample before (IMAC1) and after (IMAC2) proteolytic removal of the 8×His-SUMO tag, plus the final homogeneous hROR1-KRD protein sample after gel filtration (GF) under reducing (R) and nonreducing (NR) conditions. (d) The hROR1-KRD crystal used for high-resolution data collection mounted on the MASSIF-3 beamline at ESRF. |