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![[Figure 2]](nw5117fig2mag.jpg)
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Figure 2
Structural analysis of MPXV A42R. (a) Schematic of the β-strand and α-helical arrangement of the A42R protein. (b) A cartoon representation of the X-ray structure of the MPXV-Zaire A42R structure (PDB entry 4qwo) with β-strands in violet and α-helices in dark purple. Strands and helices in chain A are marked as indicated in (a). (c) Overlay of MPXV A42R (violet) with Homo sapiens (human) HsPFN1 (blue; PDB entry 1fil; A. A. Fedorov, T. D. Pollard & S. C. Almo, unpublished work) and human HsPFN2 (beige; PDB entry 1d1j; Nodelman et al., 1999  ). Loops are labeled according to the MPXV A42R structure as shown in (a). (d) Overlay of MPXV A42R (violet) with the structure of Bos taurus (bovine) BtPFN1 (light blue) bound to β-actin (gray; PDB entry 3ub5; Porta & Borgstahl, 2012). Loops are labeled according to the MPXV A42R structure as shown in (a). (e) Electrostatic surface projections of MPXV A42R, HsPFN1 and HsPFN2 (PDB entries 4qwo, 1fil and 1d1j, respectively). Negatively charged residues are shown in red and positively charged residues are in blue, with residues noted in the text indicated. Loops as indicated in (d) are shown for orientation of the actin-binding region. (f) Structure of MPXV A42R oriented as in (c) and colored as in (b), with residues that vary in any of the nine orthopoxviruses in the alignment in Fig. 1 indicated in yellow. Loops are as marked as in (a). |
![[Figure 2]](nw5117fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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