A partially open conformation of an androgen receptor ligand-binding domain with drug-resistance mutations

Doamekpor, S.K.; Peng, P.; Xu, R.; Ma, L.; Tong, Y.; Tong, L.

doi:10.1107/S2053230X23002224

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

IUCr IT WDC

home archive editors for authors for readers submit subscribe open access

view article

Figure 1
Drug-resistance mutations increase the soluble expression of AR LBD with antagonists. (a) Chemical structures of DHT, bicalutamide, enzalutamide, apalutamide and pruxelutamide. (b) The L702H/H875Y/F877L/T878A quadruple mutation increases the soluble expression of AR LBD in the presence of pruxelutamide (Prux) in bacteria. The W742L mutation increases the soluble expression in the presence of bicalutamide (Bic) but not pruxelutamide. The LBD is expressed as a SUMO fusion protein. Ins, insoluble fraction of cell lysate. Sol, soluble fraction after nickel affinity chromatography.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 79| Part 4| April 2023| Pages 95-104

https://doi.org/10.1107/S2053230X23002224

Follow Acta Cryst. F

Search IUCr Journals		doi		Advanced search
Author		volume	page