 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 3]](ji5030fig3mag.jpg)
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Figure 3
The role of a bound Ca2+ ion in the crystal structure of ligand-free Colchicalin. (a) A Ca2+-binding site with octahedral coordination in the crystal structure of the apoprotein. The four residues that contact the metal ion, two side chains and two main-chain carbonyl O atoms, as well as two coordinating water molecules, are depicted as sticks or small spheres. The 2Fo − Fc electron density around the Ca2+ ion is shown contoured at 5σ. (b) Fluorescence titration of Colchicalin D6.2 with colchicine in the presence or absence of 200 mM CaCl2 reveals no significant influence on the ligand affinity. |
![[Figure 3]](ji5030fig3.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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