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Figure 5
Glow discharging renders cryo-EM grids hydrophilic. (a) The commonly used commercially available Pelco Easiglow Glow Discharge Cleaning System. (b) The characteristic glow in the vacuum chamber of the glow discharger confirms the unit's function of rendering hydrophobic films hydrophilic. (c) Cartoon depiction of the effect of glow discharging. A ball of liquid (shown in blue) will not spread on a hydrophobic carbon surface. It will spread evenly over the support surface after it has been made hydrophilic by glow discharging. (d) Pictures of a 3 mm grid with carbon support film before (top) and after (bottom) glow discharging with 4 µl aqueous solution applied.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 80| Part 4| April 2024| Pages 74-81
https://doi.org/10.1107/S2053230X24002553
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