 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 1]](va5058fig1mag.jpg)
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Figure 1
Verification of DARPin–BARD1 binding by biolayer interferometry (BLI) and analytical size-exclusion chromatography (AnSEC). (a) Putative anti-BARD1 DARPin molecules were screened for BARD1 binding by loading His-tagged DARPins onto NTA Biosensors and incubating with BARD1 for 5 min and then with analyte-free buffer for 5 min. The large wavelength shifts for DARP.BARD1.01 and DARP.BARD1.02 are indicative of strong antigen binding. (b) The size-exclusion profile shows that a DARPin–BARD1 mixture (peak 3) has an altered retention time relative to BARD1 (peak 2) or the anti-BARD1 DARPin (DARP.BARD1.02; peak 1) alone. Inset: SDS–PAGE analysis of the peaks from AnSEC purification. Lane M, broad-range molecular-weight marker; lanes 1, 2 and 3 correspond to peaks 1, 2 and 3, respectively. The blue and gray arrows indicate the positions of BARD1 and DARP.BARD1.02 DARPin, respectively. |
![[Figure 1]](va5058fig1.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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