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Figure 2
Comparison of the Fab D08 and D08* knob mini-domains. (a) Rotation and translation of D08 versus D08* knob mini-domains when the heavy chains are superimposed, with gross coordinate positional shifts indicated (b). (c) Superimposition of knob mini-domain cysteines with highlighted disulfide bridges. Knob mini-domains are coloured red for D08 and magenta for D08*. (d) Visualization of r.m.s.d. differences between superimposed Fab D08 (white) and D08* (coloured blue-to-red for r.m.s.d. differences in the range 0.05–6.93 Å). Rotational and positional shifts were calculated using the RotationAxis PyMOL script (Calvo, 2014 ![]() ![]() |