Figure 2
Comparison of the Fab D08 and D08* knob mini-domains. (a) Rotation and translation of D08 versus D08* knob mini-domains when the heavy chains are superimposed, with gross coordinate positional shifts indicated (b). (c) Superimposition of knob mini-domain cysteines with highlighted disulfide bridges. Knob mini-domains are coloured red for D08 and magenta for D08*. (d) Visualization of r.m.s.d. differences between superimposed Fab D08 (white) and D08* (coloured blue-to-red for r.m.s.d. differences in the range 0.05–6.93 Å). Rotational and positional shifts were calculated using the RotationAxis PyMOL script (Calvo, 2014). R.m.s.d. differences were visualized using the ColorByRMSD PyMOL script (Shandilya et al., 2012). |