Figure 5
Interactions of the light chain with the descending strand of the BLV5B8 ultralong CDR3H. Descending β-strands of Fabs BLV5B8 (a) (Wang et al., 2013) and BLV5B8* (b) are aligned and shown in stick notation; the BLV5B8 HC is in grey, the BLV5B8 LC is in magenta and the D08 LC is in red. The D08 and BLV5B8 ultralong CDR3H loops extend beyond the top of the figure, whilst the variable domains extend below. Hydrogen bonds of interest are indicated with yellow dashed lines. In (b), the cyan dashed lines indicate the proximity of the Ile101N (BLV5B8*) carboxyl group to the amine group of the aligned Thr100B (BLV5B8). Note that the BLV5B8 heavy chain is numbered using the numbering scheme of Chothia et al. (1986). |