Figure 1
Typical format for solution-based X-ray scattering experiments of biological macromolecules. For samples that are pure and monodisperse, samples are generally loaded into a plate (right panel; check with your facility for exact formats). Matched buffer from either the concentrator eluate, purification process or dialysate must be shipped with the sample. Plates should be sealed with film and a lid. If samples are polydisperse, SEC–SAXS can be employed (left panel) and requires running buffer to be shipped along with samples. Include a 5× or 10× stock of buffer to be diluted at the facility. Solution samples should be packed carefully and in a reusable thermal shipper (bottom) that can hold temperature for several days. |