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Figure 4
Standard cryoEM sample-storage and shipping solutions. (a) Grid boxes hold four or more grids per box with lids that can be tightly secured to ensure that grids do not fall out. (b) High-capacity `pucks' can be stored in liquid nitrogen and are useful for sample organization. (c) Grid boxes can be put into 50 ml conical tubes stored in liquid nitrogen. Vent holes and a hole for a string to retrieve the tube from a dewar should be drilled into the sides of the tube. At least two knots, one outside the tube and one below the cap, should be tied so that the tube can be safely retrieved. (d) If vent holes are not drilled into the tube it can explode, causing safety issues and lost samples. |