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Figure 3
One-shot processing strategy in cryoSPARC. (a) Diagnostic images from two rounds of particle picking and 2D classification. Blob picking was first used, and templates were selected for the second round of picking using a template picker. (b) Particles belonging to classes corresponding to ApoF, β-gal, PP7 or TMV were selected and used as input for individual ab initio jobs with K = 1. (c) Output of 3D refinement jobs for all four species. Step-by-step information on jobs and settings for one-shot processing in cryoSPARC is described in Fig. 1[link].

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 80| Part 11| November 2024| Pages 320-327
https://doi.org/10.1107/S2053230X24010318
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