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Figure 1
SEC-MALS analysis of Mannitou Fab. (a) Size-exclusion chromatography profile of Mannitou Fab on a Superose 6 Increase 10/300 column upon cobalt-affinity purification. The dashed boxes indicate the portions of the eluted protein (peaks A and B) used for further analyses. (b) SDS–PAGE gel of peaks A and B and their Western blot analysis using an anti-His antibody. (c) SEC-MALS analysis of peaks A and B of the Mannitou Fab produced in HEK293 FreeStyle cells. These analyses were performed using a Superdex 200 Increase column (peak A) and a Superose 6 Increase column (peak B). The inset table shows the theoretical molecular weight and the molecular weights based on the protein content as determined by SEC-MALS analysis.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 81| Part 1| January 2025| Pages 19-29
https://doi.org/10.1107/S2053230X24012159
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