Small-angle X-ray scattering of engineered antigen-binding fragments: the case of glycosylated Fab from the Mannitou IgM antibody

Semwal, S.; Karamolegkou, M.; Flament, S.; Raouraoua, N.; Verstraete, K.; Thureau, A.; Wien, F.; Bray, F.; Savvides, S.N.; Bouckaert, J.

doi:10.1107/S2053230X24012159

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 2
SEC-SAXS profile of the Mannitou Fab sample on a Bio SEC-3 300 column. (a) Absorption at 280 nm, (b) small-angle X-ray scattering, (c) singular value decomposition and (d) evolving factor analysis. Advanced SEC-SAXS processing used the BioXTAS RAW software. (e) Guinier plot analysis for the dimer, (f) Guinier plot analysis for the monomer and (g) a dimensionless Kratky plot indicate that both proteins are well folded.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 81| Part 1| January 2025| Pages 19-29

https://doi.org/10.1107/S2053230X24012159

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