Small-angle X-ray scattering of engineered antigen-binding fragments: the case of glycosylated Fab from the Mannitou IgM antibody

Semwal, S.; Karamolegkou, M.; Flament, S.; Raouraoua, N.; Verstraete, K.; Thureau, A.; Wien, F.; Bray, F.; Savvides, S.N.; Bouckaert, J.

doi:10.1107/S2053230X24012159

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 5
Mannitou Fab fits the SAXS data optimally upon N-linked glycan conjugation. The theoretical scattering curves of the AlphaFold3 models for Mannitou Fab were calculated (solid lines) and compared with the experimental SAXS data (dots); the respective models used for the calculations are shown on the right side of each fit to the SAXS curve. (a) Highest scoring model obtained from unmodified AlphaFold3 without glycosylation, (b) multi-state model generated using GlycoSHIELD, (c) lowest scoring glycan conformer of Mannitou Fab and (d) highest scoring glycan conformer of Mannitou Fab.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 81| Part 1| January 2025| Pages 19-29

https://doi.org/10.1107/S2053230X24012159

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