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Figure 1
X-ray structures of wtFNRoxi and wtFNRred. (a) Comparison of the overall structures of oxidized (green) and reduced (yellow) R-FNR. Each model was aligned based on the positions of all Cα atoms (backbone r.m.s.d. = 0.121 Å). The green and red meshes represent the Fo(wtFNRoxi) − Fo(wtFNRred) difference map contoured at +4.0σ and −4.0σ, respectively. (b) Enlarged views around FAD/FADH2. The left panel shows the oxidized model with the difference map, while the right panel displays the reduced model with the same difference map. In the right panel, conformer A of FADH2 is shown in yellow and conformer B in pale orange. Orange arrows indicate moving water molecules. Among these, blue arrows highlight water molecules that shifted by more than 2 Å and black arrows represent newly appearing water molecules. |