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Figure 1
Effect of entB on S. maltophilia growth and siderophore production. Strain K279a (WT), the entC mutant NUS8 (entC) and the entB mutant NUS28 (entB) were inoculated into SSC medium containing 125, 150, 175 or 200 µM DIP and incubated at 37°C for 48 h. Bacterial growth was monitored spectrophotometrically (a). At 36 h, the culture supernatants were assessed for levels of reactivity in the Rioux assay, measured as net 2,3-dihydroxybenzoic acid (DHBA) equivalents (b). Asterisks indicate significant differences between the WT and mutant strains (Student's t-test; **, p < 0.005; ***, p < 0.001). Data are presented as the means and standard deviations of results from three independent experiments (n = 3 each). `ND' indicates undetectable Rioux activity. |