 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 5]](yg5013fig5mag.jpg)
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Figure 5
Examples of solution change using an automated linear gradient equilibration as described. Three different crystal pots are shown. In each panel, the intact crystals shown on the right were gradient-equilibrated and then transferred to a crystal pot containing the target solution. (a) α-Lactalbumin crystals, 0 → 25% glycerol over 50 min. (b) Thermolysin crystals, ∼2 M ammonium sulfate → water over 15 min. (c) Lysozyme crystals, 8% NaCl → 3% NaCl over 40 min. The cracked crystals shown on the left were directly transferred to the same pot with the target solution containing the gradient-equilibrated crystals. Hence, direct transfer to the target solution damages the crystals. |
![[Figure 5]](yg5013fig5.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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